Product Description
Penyangga Regenerasi, 2 x Penyangga Elusi, 10 x Penyangga Pencucian, Tabung Pengumpul 50 mL, Tabung Desalting, Tutup Tekan, Kolom Mini
Introduction
The ViraTrapTM Adenovirus maxi purification kit is designed for fast and efficient purification of recombinant Adenovirus from adenovirus transfected cell culture supernatant. Up to 4 x1012 viral particles can be purified from cell culture media of 6 to 8 T75 flasks.
Traditionally the recombinant lentivirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.
Each column can be regenerated for purifying the same adenovirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
Semua komponen dijamin setidaknya 12 bulan sejak tanggal pembelian jika disimpan sebagai berikut: Kolom mini dan tabung desalting harus disimpan pada suhu 4 ° C, dan semua bahan lainnya pada suhu RT (22-25 ° C).
Before Starting
Akrab dengan setiap langkah dengan membaca menu ini dan persiapkan semua bahan untuk prosedur.
Kit Contents
| Catalog# |
V1260-00 |
V1260-01 |
V1260-02 |
Notes |
| Preps |
2 |
4 |
10 |
|
| Maxi Columns |
1 |
2 |
5 |
Can be used twice (Store at 4°C) |
| Press-On Cap |
1 |
2 |
5 |
Store at RT |
| Desalting Tube* |
Not Included |
1 |
1 |
Can be regenarated (Store at 4°C) |
| 50 mL Collection Tube |
2 |
4 |
10 |
Store at RT |
| 10 x Wash Buffer |
6 mL |
30 mL |
60 mL |
Store at RT |
| 2 x Elution Buffer |
6 mL |
30 mL |
60 mL |
Store at RT |
| Regeneration Buffer |
6 mL |
30 mL |
60 mL |
Store at RT |
* Tabung desalting dapat dibuat ulang dan diaplikasikan pada satu jenis virus. Tabung desalting dapat dibeli dari Biomiga.
Safety Consideration
Media sel yang terinfeksi adenovirus dan virus yang dimurnikan dapat menjadi bahan yang menimbulkan bahaya hayati dan dapat menular ke manusia dan hewan. Semua protokol HARUS dilakukan di bawah kondisi kerja setidaknya Bio-Safety level 2.
Materials required but not supplied
1. ddH2O
2. PBS
3. 0.45 μm and 0.22 μm filters
4. Rack holder for columns
MORE INFO :
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Website : www.bmdstore.com
No Kantor : 021-2943 2095
No HP : 0813 1754 7882
Email : [email protected]
